The Hantaan virus M-segment glycoproteins G1 and G2 can be expressed independently
نویسندگان
چکیده
منابع مشابه
Posttranslational processing of Uukuniemi virus glycoproteins G1 and G2.
Uukuniemi virus, which matures specifically in the Golgi complex, contains two species of envelope glycoproteins, G1 (Mr, 70,000) and G2 (Mr, 65,000). These are translated as a polyprotein, p110, from an mRNA which is complementary to the medium-sized segment of the virion RNAs. By synchronized initiation of protein synthesis and pulse-labeling, it was shown that glycoprotein G1 is amino termin...
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Baculovirus and vaccinia virus vectors were used to express the small (S) and medium (M) genome segments of Hantaan virus. Expression of the complete S or M segments yielded proteins electrophoretically indistinguishable from Hantaan virus nucleocapsid protein or envelope glycoproteins (G1 and G2), and expression of portions of the M segment, encoding either G1 or G2 alone, similarly yielded pr...
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We examined whether a vesicular stomatitis virus (VSV) pseudotype bearing the hantavirus envelope glycoproteins (GPs) G1 and G2 (VSVdeltaG*HTN) could be used as a safe and effective alternative to native hantavirus. Mice were immunized with purified particles of VSVdeltaG*HTN. After the second immunization, all mice produced anti-GP antibody as detected in ELISA and a neutralization test. After...
متن کاملMolecular characterization of the Prospect Hill virus M RNA segment: a comparison with the M RNA segments of other hantaviruses.
Complementary DNA representing the genomic M RNA segment of the Prospect Hill (PH) Hantavirus was cloned and its nucleotide sequence determined. The PH virus M RNA segment is 3707 nucleotides in length and has a long open reading frame in the viral complementary-sense RNA with a coding capacity of 1142 amino acids. The predicted gene product of the PH virus M segment was compared with the corre...
متن کاملMixing of M segment DNA vaccines to Hantaan virus and Puumala virus reduces their immunogenicity in hamsters.
To determine if DNA vaccines for two hantaviruses causing hemorrhagic fever with renal syndrome, Hantaan virus and Puumala virus, are immunogenic when given in combination, we delivered them to hamsters separately or as mixtures by gene gun or by electroporation. Both vaccines elicited neutralizing antibodies when given alone but when they were delivered as a mixture, antibodies to only one of ...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1992
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.66.4.1907-1914.1992